Optimization of the cultural conditions to enhance lipase production by environmental bacteria
Abstract
The production of lipase enzymes has played an essential role in biotechnology since they are used in various environmental applications, including oil pollution treatment. The present study aims to increase the production of lipase enzymes by optimizing nutritional and environmental factors from bacteria isolated from the soil and water from different sites in Basrah province. Seven bacterial isolates out of the fifty that showed positive results in the primary screening exhibited the highest activity in the secondary screening. These isolates belong to the species including Bacillus subtilis strain QD517, Bacillus velezensis strain Bac104, Bacillus subtilis strain PK9, Bacillus cereus strain RB1, Enterobacter cloacae strain YY-2, Bacillus subtilis strain SPA N1, and Bacillus velezensis strain FJ23. The values of lipase enzyme activities of the seven isolates under normal cultivation conditions were 49 U/ml, 28 U/ml, 24 U/ml, 23 U/ml, 23 U/ml, 18 U/ml, and 18 U/ml, respectively. Nutritional and environmental factors including carbon sources, nitrogen sources, phosphorus sources, temperature, pH, and incubation period were examined in this study to increase lipase enzyme activity. The optimal environmental and nutritional conditions were 37 °C, a pH of 7, sunflower oil as the best carbon source, peptone as the best nitrogen source, NH₄H₂PO₄ as the best phosphorus supply and four days as the optimum period of incubation. The maximum lipase activity values for the seven bacterial isolates under optimal conditions were 89 U/ml, 91 U/ml, 86 U/ml, 92 U/ml, 88 U/ml, 88 U/ml, and 89 U/ml, respectively for the bacterial isolates of Bacillus velezensis strain Bac104, Bacillus subtilis strain PK9, Bacillus cereus strain RB1, Bacillus subtilis strain QD517, Bacillus subtilis strain SPA N1, Bacillus velezensis strain FJ23, and Enterobacter cloacae strain YY-2.
Full Text:
Full text PDFReferences
Abbas, S. Z.; Yong, Y; Khan, M. A.; Siddiqui, M. R.; Hakami, A. A. H.; Alshareef, S. A.; Otero, M. and Rafatullah, M. (2020). Bioflocculants produced by bacterial strains isolated from palm oil mill effluent for application in the removal of Eriochrome Black T dye from water. Polymers, 12 (7): 1545.
Adetunji, A. I. and Olaniran, A. O. (2021). Production strategies and biotechnological relevance of microbial lipases: a review. Brazilian journal of microbiology, 52(3): 1257-1269.
Al-Amery N. M. A and Alyousif, N. A. (2024). Isolation, screening and molecular characterization of feather degrading bacteria isolated from poultry soil in Basrah province. Biodiversitas, 25(9): 2560-2569.
Alhebshi, A.; Al-Sayied, F. S. and El-Hamshary, O. I. (2023). Detection and molecular characterization of lipase-producing bacteria. Egyptian Pharmaceutical Journal, 22(1): 54-66.
Al Mohaini, M., Farid, A., Muzammal, M., Ghazanfar, S., Dadrasnia, A., Alsalman, A. J. and Ismail, S. (2022). Enhancing lipase production of Bacillus salmalaya strain 139SI using different carbon sources and surfactants. Applied Microbiology, 2(1), 237-247.
Alyousif, N.A.; Al-Tamimi, W. H. and Al-sahib, M. A. A. (2022). Evaluation of the effect of various nutritional and environmental factors on biosurfactant production by Staphylococcus epidermidis. Biodiversitas, 23(7): 3533-3538.
Bashir, T.; Majeed, H. and Iftikhar, T. (2024). Isolation and Screening of Thermophillic Bacteria and its Subsequent Evaluation for Lipases Production. Pakistan Journal of Botany, 56(2): 759-764.
Fatima, S.; Faryad, A.; Ataa, A.; Joyia, F. A. and Parvaiz, A. (2021). Microbial lipase production: A deep insight into the recent advances of lipase production and purification techniques. Biotechnology and applied biochemistry, 68(3): 445-458.
Guo, C.; Zheng, R.; Cai, R.; Sun, C. and Wu, S. (2021). Characterization of two unique cold-active lipases derived from a novel deep-sea cold seep bacterium. Microorganisms, 9(4): 802.
Hasan, N. A., Nawahwi, M. Z., Yahya, N., and Othman, N. A. (2018). Identification and optimization of lipase producing bacteria from palm oil contaminated waste. Journal of Fundamental and Applied Sciences, 10(2S), 300-310.
Hemlata, B.; Uzma, Z. and Tukaram, K. (2016). Substrate kinetics of thiol activated hyperthermostable alkaline lipase of Bacillus sonorensis 4R and its application in bio-detergent formulation. Biocatalysis and Agricultural Biotechnology, 8: 104-111.
Iboyo, A. E., Asitok, A. D., Ekpenyong, M. G., and Antai, S. P. (2017). Selection of Enterobacter cloacae strain POPE6 for fermentative production of extracellular lipase on palm kernel oil processing effluent. Int J Sci, 6, 1-17.
Ikhwani, A. Z. N., Idris, I., Elfirta, R. R., and Ferdian, P. R. (2024). Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486. Journal of the Turkish Chemical Society Section A: Chemistry, 11(2), 397-404
Ilesanmi, O. I.; Adekunle, A. E.; Omolaiye, J. A.; Olorode, E. M. and Ogunkanmi, A. L. (2020). Isolation, optimization and molecular characterization of lipase producing bacteria from contaminated soil. Scientific African, 8: e00279.
Issa, H. K.; Abou Dobara, M. I.; El-Sayed, A. K. and El-Bana, M. I. (2024). Check for updates Bioprospecting of Lipase Producing Bacteria Isolated Form Oil Contaminated Sites of Port Said, Egypt. In Proceedings of The First International Conference on Green Sciences: Environmental Science for Green and Sustainable Environment (p. 269). Springer Nature.
Jamilu, H.; Ibrahim, A. H., and Abdullahi, S. Z. (2022). Isolation, optimization and characterization of lipase producing bacteria from abbatoir soil. International Journal of Scientific Advances, 3: 2708-7972.
Mazhar, H.; Ullah, I.; Ali, U.; Abbas, N.; Hussain, Z.; Ali, S. S. and Zhu, H. (2023). Optimization of low-cost solid-state fermentation media for the production of thermostable lipases using agro-industrial residues as substrate in culture of Bacillus amyloliquefaciens. Biocatalysis and Agricultural Biotechnology, 47: 102559.
Mobarak-Qamsari, E.; Kasra-Kermanshahi, R. and Moosavi-Nejad, Z. (2011). Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110. Iranian journal of microbiology, 3(2): 92.
Murtius, W. S., Hari, P. D., and Putri, I. N. (2022, July). The Effect of Incubation Time to The Activity of Lipase Produced by Bacillus thuringiensis on Coconut (Cocos nucifera L.) Dregs. In IOP Conference Series: Earth and Environmental Science (Vol. 1059, No. 1, p. 012076). IOP Publishing.
Pham, V. H. T.; Kim, J.; Chang, S. and Chung, W. (2021). Investigation of lipolytic-secreting bacteria from an artificially polluted soil using a modified culture method and optimization of their lipase production. Microorganisms, 9(12): 2590.
Rasmey, A. H. M.; Aboseidah, A. A.; Gaber, S. and Mahran, F. (2017). Characterization and optimization of lipase activity produced by Pseudomonas monteilli 2403-KY120354 isolated from ground beef. African Journal of Biotechnology, 16(2): 96-105.
Sagar, K.; Bashir, Y.; Phukan, M. M. and Konwar, B. K. (2013). Isolation of lipolytic bacteria from waste contaminated soil: A study with regard to process optimization for lipase. International Journal of Scientific and Technology Research, 2(10): 214-218.
Snellman, E. A.; Sullivan, E. R. and Colwell, R. R. (2002). Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG‐1. European Journal of Biochemistry, 269(23), 5771-.9775.
Yao, W.; Liu, K.; Liu, H.; Jiang, Y.; Wang, R.; Wang, W. and Wang, T. (2021). A valuable product of microbial cell factories: Microbial lipase. Frontiers in Microbiology, 12: 773347.
Zhao, J., Ma, M.; Zeng, Z., Yu, P.; Gong, D. and Deng, S. (2021). Production, purification and biochemical characterization of a novel lipase from a newly identified lipolytic bacterium Staphylococcus caprae NCU S6. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1): 249-257.
Refbacks
- There are currently no refbacks.
Editorial Office:
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya
Jl. Palembang-Prabumulih Km.35 Indralaya Ogan Ilir Sumatera Selatan 30662
IJFAC by Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License