Synthesis of Ag/CeO2 Nanocomposites with Noni Leaf Extract and Its Potential Antibacterial Properties

Laellia Denada, Poedji Loekitowati Hariani, Nabila Aprianti

Abstract


This study aims to perform green synthesis of Ag/CeO2 nanocomposites using noni leaf extract and to test their potential antibacterial activity. The synthesis results were characterised using X-ray Diffraction (XRD) to determine crystallite size, Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDX) to observe morphology and elemental composition, and Fourier Transform Infrared Spectroscopy (FTIR) to identify functional groups. The XRD characterization results showed the formation of typical Ag/CeO2 peaks with an average crystallite size of 15,28 nm. SEM analysis revealed morphology in the form of aggregates with varying sizes. EDX results confirmed the presence of Ag, Ce, and O elements. FTIR analysis showed the presence of typical Ce–O group absorption bands, which supports the successful synthesis of Ag/CeO2. The synthesized nanocomposites were then tested for their antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria. Antibacterial activity tests showed the formation of an 8 mm zone of inhibition (ZOI) at a concentration of 5000 ppm, demonstrating the potential of the synthesized green Ag/CeO2 as an antibacterial agent. This study confirms that the use of noni leaf extract as a reducing and stabilizing agent is an environmentally friendly approach and has potential for environmental applications.

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References


K. Negi, A. Umar, M. S. Chauhan, and M. S. Akhtar, “Ag/CeO2 nanostructured materials for enhanced photocatalytic and antibacterial applications,” Ceram. Int., vol. 45, no. 16, pp. 20509–20517, Nov. 2019, doi: 10.1016/J.CERAMINT.2019.07.030.

R. Feizollah and A. Meshkini, “Photochemically enhanced antibacterial activity of polydopamine-coated CeO2@Ag nanocomposites: Surface and colloidal engineering for wound pathogen treatment,” Colloids Surfaces A Physicochem. Eng. Asp., vol. 725, no. P1, p. 137641, 2025, doi: 10.1016/j.colsurfa.2025.137641.

Y. Amangelsin, Y. Semenova, M. Dadar, M. Aljofan, and G. Bjørklund, “The Impact of Tetracycline Pollution on the Aquatic Environment and Removal Strategies,” Antibiotics, vol. 12, no. 3, pp. 1–15, 2023, doi: 10.3390/antibiotics12030440.

Y. Yang et al., “Adsorption properties of noble-metal (Ag, Rh, or Au)-doped CeO2(1 1 0) to CO: A DFT + U study,” Comput. Mater. Sci., vol. 231, no. May 2023, p. 112543, 2024, doi: 10.1016/j.commatsci.2023.112543.

F. B. Kayani, S. Rafique, R. Akram, M. Hussain, K. Raja, and J. S. Khan, “Fabrication of novel chitosan@Ag/CeO2 hybrid nanocomposites for the study of antibacterial activity,” Phys. E Low-Dimensional Syst. Nanostructures, vol. 149, no. February, p. 115683, 2023, doi: 10.1016/j.physe.2023.115683.

A. Ahmad, M. S. Javed, S. Khan, T. M. Almutairi, A. A. A. Mohammed, and R. Luque, “Green synthesized Ag decorated CeO2 nanoparticles: Efficient photocatalysts and potential antibacterial agents.,” Chemosphere, vol. 310, p. 136841, Jan. 2023, doi: 10.1016/J.CHEMOSPHERE.2022.136841.

K. Vanasundari, P. Ponnarasi, and G. Mahalakshmi, “A green approach to synthesis of Ag-doped CeO2 nanorods embedded reduced graphene oxide nanocomposite for excellent photocatalytic and antimicrobial activity,” Inorg. Chem. Commun., vol. 165, no. May, p. 112523, 2024, doi: 10.1016/j.inoche.2024.112523.

M. H. Ali, S. K. Dutta, M. S. Sultana, A. Habib, and P. K. Dhar, “Green synthesized CeO2 nanoparticles-based chitosan/PVA composite films: Enhanced antimicrobial activities and mechanical properties for edible berry tomato preservation.,” Int. J. Biol. Macromol., vol. 280, p. 135976, Nov. 2024, doi: 10.1016/J.IJBIOMAC.2024.135976.

J. Sukumaran, R. Venkatesan, M. Priya, and S. C. Kim, “Eco-friendly synthesis of CeO2 nanoparticles using Morinda citrifolia L. leaf extracts: Evaluation of structural, antibacterial, and anti-inflammatory activity,” Inorg. Chem. Commun., vol. 170, no. P3, p. 113411, 2024, doi: 10.1016/j.inoche.2024.113411.

M. B. Nugroho, A. R. Affandi, U. Rini, and F. Nurdyansyah, “Efek Jenis Pelarut Terhadap Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Daun Mengkudu (Morinda citrifolia L),” Snse Viii, vol. 1, no. 1, pp. 91–97, 2022.

M. R. Priamsari and N. A. Yuniawati, “Skrining Fitokimia dan Aktivitas Penyembuhan Luka Bakar Ekstrak Etanolik Morinda Citrifolia L. pada Kulit Kelinci (Oryctolagus Cuniculus) Phytochemical Screening and Activity of Ethanolic Leaves Extract Morinda Citrifolia L. Against Healing Burn in Rabbit,” J. Pharm., vol. 8, no. 1, pp. 22–28, 2019.

P. Vinitha, M. V Arularasu, and R. Vignesh, “Chemistry of Inorganic Materials A review on green synthesis and applications of CeO2 nanomaterials – An eco-friendly approach,” Chem. Inorg. Mater., vol. 5, no. December 2024, p. 100084, 2025, doi: 10.1016/j.cinorg.2024.100084.

N. G. De La Cruz-Sánchez et al., “Antibacterial activity of Morinda citrifolia Linneo seeds against Methicillin-Resistant Staphylococcus spp,” Microb. Pathog., vol. 128, no. October 2018, pp. 347–353, 2019, doi: 10.1016/j.micpath.2019.01.030.

S. M. M. Balachandramohan, K. G. Kumar, and M. S. R. P. Sasikumar, “Green Synthesis of Silver Oxide Nanoparticles Using Plectranthus amboinicus and Solanum trilobatum Extracts as an Eco ‑ friendly Approach : Characterization and Antibacterial Properties,” J. Inorg. Organomet. Polym. Mater., no. 0123456789, 2024, doi: 10.1007/s10904-024-03030-6.

M. Arunpandian, T. H. Oh, and G. Sriram, “Breakthrough in High-Efficiency Photocatalytic Degradation of Acebutolol by Advanced Binary CeO2-MnO2 Oxide System,” Molecules, vol. 29, no. 12, 2024, doi: 10.3390/molecules29122854.

Y. Yulizar, S. Juliyanto, Sudirman, D. O. B. Apriandanu, and R. M. Surya, “Novel sol-gel synthesis of CeO2 nanoparticles using Morinda citrifolia L. fruit extracts: Structural and optical analysis,” J. Mol. Struct., vol. 1231, p. 129904, 2021, doi: 10.1016/j.molstruc.2021.129904.

K. M. Elattar et al., “Eco-friendly synthesis of Ag/CeO2 and CuO/CeO2 nanocomposites using Curcuma longa extract and assessment of their antioxidant, antifungal, and cytotoxic activities,” RSC Adv., vol. 15, no. 16, pp. 12100–12116, 2025, doi: 10.1039/d5ra00739a.

S. H. Alrefaee et al., “Rosmarinus officinalis-based Ag/SiO2 and CeO2-Ag/SiO2 core-shell nanocomposites: A green approach to phytochemical analyses, molecular docking, antioxidant and antimicrobial applications with enhanced biocompatibility,” Results Eng., vol. 24, no. November, p. 103478, 2024, doi: 10.1016/j.rineng.2024.103478.

A. Rani et al., “Antibacterial and photocatalytic properties of Ag-doped CeO2:BaO synthesized by simple solution combustion method,” Mater. Lett., vol. 353, no. September, p. 135270, 2023, doi: 10.1016/j.matlet.2023.135270.

A. Ahmad, M. S. Javed, S. Khan, T. M. Almutairi, A. A. A. Mohammed, and R. Luque, “Green synthesized Ag decorated CeO2 nanoparticles: Efficient photocatalysts and potential antibacterial agents.,” Chemosphere, vol. 310, no. October 2022, p. 136841, 2023, doi: 10.1016/j.chemosphere.2022.136841.

A. A. Alanazi, W. E. I. A. Saber, M. A. AlDamen, and K. M. Elattar, “Green synthesis, characterization, and multifunctional applications of Ag@CeO2 and Ag@CeO2-pullulan nanocomposites for dye degradation, antioxidant, and antifungal activities,” Int. J. Biol. Macromol., vol. 280, no. P4, p. 135862, 2024, doi: 10.1016/j.ijbiomac.2024.135862.

N. Iqbal et al., “Interrelationships between the structural, spectroscopic, and antibacterial properties of nanoscale (< 50 nm) cerium oxides,” Sci. Rep., vol. 11, no. 1, pp. 1–15, 2021, doi: 10.1038/s41598-021-00222-9.


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