Ecoenzyme Characterization of Fruit Peel Waste Mixture and Test of Antibacterial Activity against Bacteria Causing Dental Caries
Abstract
Ecoenzyme is a product of facultative aerobic fermentation for 3 months from organic fruit and vegetable waste. In this study, ecoenzyme was made from a mixture of fruit peel waste (papaya, orange and pineapple) with the addition of 6 liters of water. Ecoenzyme products were characterized by organoleptic properties, chemical composition, enzyme activity and antibacterial activity tests against Lactobacillus acidophilus and Streptococcus mutans bacteria that cause dental caries. The results of the study show that the ecoenzyme product had a dominant orange peel sour flavor, was cloudy brown in color with a volume of 8.4 liters from 6 liters of added water. The characterization results depict that the chemical composition of ecoenzyme contained acetic acid and lactic acid with a total acid content of 2.26%, 40.45 mg/mL protein, and secondary metabolites in the form of alkaloids, flavonoids, and saponins. The activity of several enzymes from ecoenzyme was protease 0.0246 U/mL, amylase 0.0032 U/mL and lipase 5 U/mL. Antibacterial activity against bacteria that cause dental caries is included in the category of very strong against S. mutans at a concentration of 40 (% v/v) and moderate against L. acidophilus with inhibition zone diameters of 23.33 mm and 8.23 mm respectively. The Mininum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MKC) values of ecoenzyme against S. mutans and L. acidophilus bacteria were 2500 ppm and 5000 ppm respectively.
Keywords: ecoenzyme, fruit-peel waste, L.acidophilus, S.mutans, antibacterialFull Text:
Full text PDFReferences
G. G. Gumilar, “Ecoenzyme Production, Characteristics, and Applications: A Review,” J. Kartika Kim., vol. 6, no. 1, pp. 45–59, 2023, doi: 10.26874/jkk.v6i1.186.
A. Z.,Salsabila, “Karakterisasi Biokimia Ekoenzim dari Kulit Pisang Kepok Manado (Musa paradisiaca var. formatypica) Muda dan Daya Hambatnya Pada Fusarium sp. dan Xanthomonas campestris”. Universitas Lampung. 2023.
S. N. Mardiyah, “Isolasi dan Karakterisasi Enzim Amilase dari Ekoenzim”. Universitas Islam Negeri Sunan Gunung Djati Bandung. 2021.
N. Lubis and S. Wardani, “Antibacterial Activity of Ecoenzymes with Various Dilutions Against Escherichia Coli,” pp. 122–127.
E. Pratamadina and Wikaningrum. “Potensi Penggunaan Eco Enzyme sebagai Biokatalis dalam Penguraian Minyak dan Lemak pada Air Limbah Domestik. Serambi Engineering”. Vol. 7(4), pp. 3924-3932. 2022.
E. Fibryanto, and L. Santoso, “Mouthwashes: a review on its efficacy in preventing dental caries”. Jurnal Kedokteran Gigi Terpadu. 5(1): 91-93. 2023.
E. Antony, L Mitchell, L Nettenstrom, and Speckhard D. 2000. When A + B ≠ B + A. J Chem Educ. 77(9): 1180-1181.
S. B. Mohammed, “Effects and Effectiveness of Cavity Disinfectants in Operative Dentistry: A Literature Review”. The Journal of Contemprary Dental Practice. 17(10):867-879. 2016.
H. A. K. Mavani, In M. T., Lishen W., Hsu Z. Y., Alida M., Rohi A. G., and Edmond H.N.P. “Antimicrobial Efficacy of Fruit Peels Eco-Enzyme agains t Enterococcus faecalis: An In Vitro Study”. International Journal of Environmental Research and Public Health. 17(1):1-2.2020
M. A. Dewi., Anugrah, R., and Nurfitri, Y. A. Uji Aktivitas Antibakteri Ekoenzim terhadap Escherichia Coli Dan Shigella Dysenteriae. Seminar Nasional Farmasi (SNIFA)2 UNJANI .1(1):60-68. 2016
R. Y Viza, “Uji Organoleptik ecoenzyme dari kulit buah”. Bioedusains: J.Pendidikan Biol. Sains. Vol. 5(1), pp. 24-30. 2022
M. Gorreti, and M. Purwanto, “Perbandingan Analisa Kadar Protein Terlarut dengan Berbagai Metode Spektroskopi UV-Visble”. Jurnal Ilmiah Sains & Teknologi. Vol 7 (1), pp. 1–17. 2014.
I. Sulistyarini, and D. A. Sulistyarini, and Wicaksono, T. A. Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga. Jurnal Ilmiah Cendekia Eksaktak. vol.1 (1), pp. 56-62.2020
R. Nugrahani, Y. Andayani, and A. Hakim, “Skrining Fitokimia Dari Ekstrak Buah Buncis (Phaseolus vulgaris L) Dalam Sediaan Bubuk”. Jurnal Pendidikan IPA.vol. 2 (1), pp. 96-103. 2016
Y. Andriani, H. Mohamad, B. Kesaven, M. I. Abdullah, and H. Amir, “Phytochemical, Antibacteria and Antibiofilm Activities Against Pseudomonas aeruginosa of Various Extracts and Fractions of Mangrove Associated, Hibiscus tiliaceus”. Journal of Sustainability Science & Management (JSSM). 12(2):45-51.2017
Refbacks
- There are currently no refbacks.
Editorial Office:
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya
Jl. Palembang-Prabumulih Km.35 Indralaya Ogan Ilir Sumatera Selatan 30662
IJFAC by Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License