Sweet flag (Acorus calamus L.) rhizomes chromatography column isolates potential as rich antioxidant compounds

Suci Amalia, Lili Sunarti Hasibuan, Ninit Arinda Arianti, A Ghanaim Fasya, Diana Chandra Dewi, Deva Krisna Kadarani


the unavailability of local materials in purposing of independently manufacturing pharmaceutical products. One of plants which can be used for raw materials in the biopharmaceuticals, sweet flag (Acorus calamus L.) that is rich in bioactive compounds such as flavonoids, alkaloids, phenolic compounds, tannins, steroids, saponins, glycosides and terpenoids. Flavonoids are the majority bioactive compounds in sweet flag rhizomes, which leads this study to isolate the flavonoids to identify as antioxidant agent for biopharmaceutical products development later. This study determined the antioxidant activity of sweet flag rhizomes as its crude extract, ethyl acetate fraction, and column isolate. Decreasing range of phytochemical compounds was remained only flavonoid that was in the column isolate.  Liquid chromatography Mass Spectrometry (LC-MS) in column isolates was identified isorhamnetin, quercetin, kaempferol, genistein, and glycitein. Additionally, this study aims the data that column isolate has the highest purity level with the least inhibitory concentration 50%  (IC50) and the strongest antioxidant activity.

Keywords: Jeringau Rhizome, Acorus calamus, Column Isolate, Flavonoids, Antioxidants 

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Aronson, JK. 2016. Meyler’s Side Effects of Drugs Sixteenth Edition, International Encyclopedia of Adverse Drug Reaction and Interactions. Elsevier Science.

Miao, J., Shi, R., Li, C., Li, X., Chen, Q. 206. Chapter 88- Sweet Flag (Acorus calamus) Oils in Essential Oils in Food Preservation, Flavor and Safety. Academic Press. 775-782.

Nair, A., Chattopadhyay, D., Saha, B. 2019. Chapter 17 – Plant-derived Immunomodulator in New Look to Phytomedicine Advancements in Herbal products as Novel Drug Leads. Academic Press. 435-499.

Zhao et al. 2023. Ethnic, Botanic, Phytochemistry and Pharmacology of the Acorus L. Genus: A review. Molecules. 28(20):7117. DOI: 10.3390/molecules28207117

Gouot, J.C., Smith, J.P., Holzapfel, B.P., Walker, A.R., Barril, C. 2019. Grape Berry Flavonoid: A Review of Their Biochemical Responses to High and Extreme High Temperatures. Journal of Experimental Botany. 70(2).

Obieogu, K. Nwosu., Dzarma, G.W. 2022. Effect of Dilute Acid Pretreatment on Melon Seed Husk Sructure. Elsevier.

Winterton, Neil. 2021. The Green Solvent: A Critical Perspective. Springer.

D, P.M & Jha, Dr. Cha. V. 2021. Qualitative and Quantitative Phytochemical Screening of Three Plants Stem Bark and Leaves From Sapotaceae Family. International Journal of Multidisciplinary Educational Research. 10(6).

Fasya, A. G., Tyas, A. P., Mubarokah, F. A., Ningsih, R., & Madjid, A. D. R. (2018). Variasi Diameter Kolom Dan Rasio Sampel-Silika Pada Isolasi Steroid Dan Triterpenoid Alga Merah Eucheuma Cottonii Dengan Kromatografi Kolom Basah. Alchemy. 6(2).

Munajad, A., Subroto, C., Suwarno. 2018. Fourier Transform Infrared (FTIR) Spectroscopy Analysis of Transformer Paper in Mineral Oil-Paper Composite Insulation Under Accelerated. MDPI. 11(2).

Fanelli, S., Zimmermann, A., Totoli, E.G., Salgado, H.R.N. 2018. FTIR Spectrophotometry as a Green Tool for Quantitative Analysis of Drugs: Practical Application to Amoxicillin. Journal of Chemistry.

Yunita, E., Yulianto, D., Fatimah, S., Firanita, T. 2020. Validation of UV-Vis Spectrophotometric Method of Quercetin in Ethanol Extract of Tamarind Leaf. Journal of Fundamental and Applied Pharmaceutical Science.

Sastrohamidjojo. 1991. Spektroskopi. Yogyakarta: Liberty

Sibaba, S.V., Kumar, M.S., Pandiyan, P.S. 2016. Mini Review on LC/MS Techniques. World Journal of Pharmacy and Pharmaceutical Science. 5(4).

Shan Ku, Y., Sin Ng, M., Shan Ceng, S. Yi Lo, A.W., Xiao, Z., Sun Shin, T., Chung, G., Ming Lam, H. 2020. Understanding the Composition, Biosynthesis, Accumulation and Transport of Flavonoids in Crops for the Promotion of Crops as Healty Sources of Flavonoids for Human Consumption. MDPI.12(6).

Mailuhu, M., Runtuwene, M. R. J., Koleangan, H. S. .J 2017. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Kulit Batang Soyogik (Saurauia) bracteosa DC). Chemistry Program. 10(1).

Dolya, V.S. 2002. Практикум по фармакогнозии: учебное пособие для студ : Изд-во НФАУ, Золотые страницы.

Lahlminghlui, K & Jagetia, G.C. 2018. Evaluation of the Free- Radical Scavenging and Antioxidant Activities of Chilauni, Schima wallichii Korth in Vitro. National Library of Medicine. 4(2).

DOI: http://dx.doi.org/10.24845/ijfac.v9.i2.66


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