Antibacterial Activity and Structure Elucidation of Salicin from Stem Bark of Salix tetrasperma ROXB.

Sari Ramadhani, Adlis Santoni, Mai Efdi

Abstract


Salix tetrasperma Roxb. (Family Salicacaeae) is a plant that used as traditional medicine for anti-inflammatory, analgesic, reduces fever, and itching medicine. In this study was carried out extraction, isolation, structure elucidation of salicin from Salix tetrasperma Roxb. stem bark and it’s antibacterial activity. The extraction method was used the maceration method by n-hexane, ethyl acetate, and methanol solvents. Isolation of compound from ethyl acetate extract of Salix tetrasperma Roxb. stem bark using chromatography methods and obtained white solid (15 mg). The structure was elucidated using spectroscopic analysis, including Ultraviolet (UV), Infrared (IR), Nuclear Magnetic Resonance (NMR) and comparative literature, identified as salicin compound with molecule formula C13H18O7. Antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus bacteria using disk diffusion method. This compound has a great an antibacterial activity against Staphylococcus aureus bacteria with clear zone diameter of 10.2 ± 0.3 mm. This shows that the Salix tetrasperma Roxb. stem bark has great potential as a source of antibacterial compound


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References


K. R. Kirtikar and B. D. Basu, Indian Medicinal Plants. Dehradun: Lalit Mohan Basu, pp. 2362-2363, 2005.

B. Schmid et al., “Efficacy and tolerability of a standardized willow bark extract in patients with osteoarthritis: randomized placebo-controlled, double blind clinical trial.,” Phytother. Res., vol. 15, no. 4, pp. 344–50, Jun. 2001.

R. Valsaraj, P. Pushpangadan, U. W. Smitt, A. Adsersen, and U. Nyman, “Antimicrobial screening of selected medicinal plants from India.,” J. Ethnopharmacol., vol. 58, no. 2, pp. 75–83, Oct. 1997.

A. El-Shazly, A. El-Sayed, and E. Fikrey, “Bioactive secondary metabolites from Salix tetrasperma Roxb.,” Zeitschrift fur Naturforschung. C, vol. 67, no. 7–8, pp. 353–359, 2012.

E. A. El-Wakil, E.-S. S. Abdel-Hameed, M. M. El-Sayed, and E. E. Abdel-Lateef, “Identification of the chemical composition of the methanolic extract of Salix tetrasperma Roxb. using LC-ESI-MS and evaluation its potential as antioxidant agent,” Der Pharma Chem., vol. 7, no. 2, pp. 168–177, 2015.

Y. Fahrimal, A. Rafina, A. Azhar, and D. Nuzul Asmilia, “Blood Profile of Rats (Rattus norvegicus) Infected with Trypanosoma evansi Treated with Willow Tree Bark Extract (Salix tetrasperma Roxb),” J. Kedokt. Hewan, vol. 8, no. 2, pp. 164–168, 2014.

C. R. Raj, GK. Dash, M. Sumanta, and S. Parhi, “Studies on the Hypoglycaemic Activity of The Bark of Salix tetrasperma Roxb.,” Int. J. Drug Dev. Res., vol. 2, no. 4, 2009.

M. Sumanta, H. Ramana, and C. Rishi Raj, “Studies on Diuretic and Laxative Activity of the Bark of Salix Tetrasperma Roxburgh,” Int. Res. J. Pharm., vol. 1, no. 1, pp. 145–149, 2010

M. S. Islam et al., “Antibacterial, Insecticidal and in Vivocytotoxicity Activities of Salix Tetrasperma,” Int. J. Pharm. Sci. Res., vol. 2, no. 8, pp. 2103–2108, 2011.

S. M. Moustafa, B. M. Menshawi, G. M. Wassel, K. Mahmoud, and M. Mounier, “Screening of some Plants in Egypt for their Cytotoxicity against four Human Cancer cell lines,” Int. J. PharmTech Res., vol. 6, no. 3, pp. 1074–1084, 2014.

E. S. Abdel-Hameed, H. A. El-Nahas, and S. A. Abo-Sedera, “Antischistosomal and Antimicrobial Activities of Some Egyptian Plant Species,” Pharm. Biol., vol. 46, no. 9, pp. 626–633, Jan. 2008.

S. A. Deepak, G. Oros, S. G. Sathyanarayana, H. S. Shetty, and S. Sashikanth, “Antisporulant Activity of Watery Extracts of Plants against Sclerospora graminicola causing Downy Mildew Disease of Pearl Millet,” Am. J. Agric. Biol. Sci., vol. 2, no. 1, pp. 36–42, 2007.

T. Petkar, A. Siddiqha, and P. T. Kekuda, “Evaluation of antifungal activity of some plants against seed-borne fungi,” Sch. J. Agric. Vet. Sci., vol. 4, no. 4, 2017.

P. T. Kekuda, K.S. Vinayaka and P. S. Kumar, “Antimicrobial activity of Salix tetrasperma Roxb. (Salicaceae),” Int. J. Herb. Med., vol. 5, no. 5, pp. 192–195, 2017.

X. Pan, F. Chen, T. Wu, H. Tang, and Z. Zhao, “The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT,” Food Control, vol. 20, no. 6, pp. 598–602, Jun. 2009.

C. Noleto-Dias, J. L. Ward, A. Bellisai, C. Lomax, and M. H. Beale, “Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine,” Fitoterapia, vol. 127, pp. 166–172, Jun. 2018.

O. Stefanović, I. Radojević, S. Vasić, and L. Čomić, “Antibacterial Activity of Naturally Occurring Compounds from Selected Plants,” in Antimicrobial Agents, V. Bobbarala, Ed. London: IntechOpen, 2012, pp. 1–25.

M. O. Soares, A. F. Vinha, F. Coutinho, and P. C. Pires, “Antimicrobial natural products,” in Microbial Pathogens and Strategies for combating them: Science, Technology and Education, A. Mendez-Vilas, Ed. Elche: Formatex, 2013, pp. 915–916.

D. Savoia, “Plant-derived antimicrobial compounds: alternatives to antibiotics,” Future Microbiol., vol. 7, no. 8, pp. 979–990, Aug. 2012.




DOI: http://dx.doi.org/10.24845/ijfac.v4.i2.47

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