Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract

Sofia Sandi, Miksusanti Miksusanti, Meisji Liana Sari, Eli Sahara, Adi Supriyadi, Nuni Gofar, Asmak Asmak

Abstract


Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.

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References


Jannah, RF. 2017. Karakteristik Bakteri Asam Laktat sebagai Probiotik pada Silase Rumput Kumpai Tembaga dan Legum Kemon Air. Skripsi Program Studi Peternakan, Pertanian. Universitas Sriwijaya

Hardiningsih, R., Napitupulu, R.N.R dan Yulinery, T., 2006. Isolasi dan uji resistensi beberapa isolat lactobacillus pada pH rendah. Biodiversitas 7(1): 15-17

Steel, RGD. dan Torrie, JH. 1991. Prinsip dan Prosedur Statistika. Terjemahan : M. Syah. PT. Gramedia. Jakarta.

Salminen, S., Wright, AV., Ouwehand A. 2004. Lactic Acid Bacteria. New York: Marckel Dekker.

Harimurti, S., Endang S.R., Nasroedin dan Kurniasih. 2007. Bakteri Asam Laktat dari Intestin Ayam Sebagai Agensia Probiotik. Animal Production. 9 (2): 82 – 91.

Drasar, B.S. dan P.A. Barrow. 1985. Intestinal Microbiology. American Society for Microbiology. Washington.

Gusminarni. 2010. Aktivitas Penghambatan Bakteri Asal Saluran Pencernaan Ayam Broiler terhadap Escherichia coli dan Salmonella spp Pada Berbagai Media, Aeras, pH dan Suhu. Thesis. Pascasarjana Institut Pertanian Bogor .

Nannen, N.L. dan R.W. Hutkins. 1991. Intracellular pH Effects in Lactic Acid Bacteria. J. Dairy Sci. 74 (3), 741-746.

Ali, AIM., Sandi, S. Muhakka. dan Riswandi. 2012. Kualitas Hijauan Pakan di Rawa Lebak Padang Penggembalaan Kerbau Pampangan. Prosiding InSINAS 2012.

Efendi, AH. 2009. Kajian Daya Hambat dan Daya Simpan Bakteri Asam Laktat Silase Ransum Komplit dengan dan Tanpa Kapsulasi. [tesis]. Bogor (ID). IPB

Manin, F. 2010. Potensi Lactobacillus acidophilus dan Lactobacillus fermentum dari Saluran Pencernaan Ayam Buras Asal Lahan Gambut sebagai Sumber Probiotik. Jurnal Ilmiah Ilmu-Ilmu Peternakan. Vol. 8 (5):221-228

Jannah, SR. 2014. Analisis Keragaman Bakteri Asam Laktat dari Saluran Pencernaan Ayam Cemani berdasarkan Gen 16s rRNA dan Potensi Penggunaannya sebagai Probiotik. Tesis Institut Pertanian Bogor.

Saskia 2014

Harahap, AE. 2014. Simulasi Bakteri Asam Laktat yang Diisolasi Dari Silase Daun Pelepah Sawit pada Saluran Pencernaan Ayam. Jurnal peternakan, vol 11 no 2 september 2014 (43 – 47) issn 1829 – 8729

Sjofjan, O., Natsir, MH. Dan Ardiati, T. 2015. Efek Penggunaan ProbiotikKultur Campuran dalam Air Minum terhadap Karakteristik dan Mikroflora Usus Ayam Petelur. Jurnal Ilmiah Ilmu Biologi.Vol. 1 (1), 52-58.

Mojgani, N. and C. Amirnia. 2007. Kinetics of Growth and bacteriocin production in L. casei RN 78 isolated from a dairy sample in IR Iran. International journal of Dairy science 2(1): 1-12

Harahap, AE. 2009. Kajian Daya Hambat dan Daya Simpan Bakteri Asam Laktat Silase Ransum Komplit Dengan dan Tanpa Kapsulasi. Institusi Pertanian Bogor. Bogor

Putri, WDR., Haryadi., Marseno, DW. Dan Cahyanto, MN. 2012. Isolasi dan Karakterisasi Bakteri Asam Laktat Amilolitik selama Fermentasi Growol, Makanan Tradisional Indonesia. Jurnal Teknologi Pertanian, Vol. 13 No. 1. Hal 52-60.

Nur, H. S. 2005. Pembentukan Asam Organik oleh Isolat Bakteri Asam Laktat Pada Media Ekstrak Daging Buah Durian (Durio zibethinus Murr.). Bioscientiae, 2(1): 15-24




DOI: http://dx.doi.org/10.24845/ijfac.v4.i1.15

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