Bioremediation of Spent Bleaching Earth (SBE) Waste Using Lipolytic Fungi

Riezkatama Menangcaye, Adipati Napoleon, Bambang Yudono

Abstract


The purpose of this research was to obtain the effectiveness level of bioremediation process of oil residue on SBE waste by using the isolates of lipolytic fungus Aspergilus fumigatus, Cylindrocladium sp and Fumago sp. This research was conducted by using completely randomized factorial design with 3 factors of treatment which consist of nutrient ratio (N:P:K) that were divided into three levels, namely n1=A(7:1.5:0.5), n2=B(14:3:1), n3=C(21:6:1.5). The humidity were divided into 3 levels treatment i.e. k1 (90%), k2 (80%), k3 (70%) and time that were divided into four levels, namely w1 (week 1), w2 (week 2), w3 (week 3), and w4 (week 4) with fixed pH i.e. pH 6, so that were obtained 36 combination of treatment and for each treatment combination repeated 3 times. Sampling of SBE waste was conducted in one cooking oil factory in South Sumatera using randomized sampling method as much as 24.000 g. The results showed that 80% humidity and nutrient A as the best treatment for lipolytic fungus consortium performance to degrade the SBE waste with the average percentage of oil degradation as much as 74.83%. 80% humidity and nutrient A was an effective interaction in affecting the percentage of oil degradation.


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References


S. K. Loh, S. James, M. Ngatiman, K. Y. Cheong, Y. M. Choo, and W. S. Lim, “Enhancement of palm oil refinery waste -Spent bleaching earth (SBE) into bio organic fertilizer and their effects on crop biomass

growth,” Ind. Crops Prod., vol. 49, pp. 775–781, 2013.

O. Wang and J. Coates, “Biotechnological Applications of Microbial (Per)chlorate Reduction,” Microorganisms, vol. 5, no. 4, p. 76, 2017.

A. A. Juwarkar, S. K. Singh, and A. Mudhoo, “A comprehensive overview of elements in bioremediation,” Rev. Environ. Sci. Biotechnol., vol. 9, no. 3, pp. 215–288, 2010.

M. Piotrowska, A. Otlewska, K. Rajkowska, A. Koziro, M. Hachułka, P. N. Krawczy, G. J. Wolski, B. Gutarowska, A.K. Styczyn´ ska, and A. Z. ydzik-Białek, “Abiotic Determinants of the Historical Buildings Biodeterioration in the Former Auschwitz II ’ Birkenau Concentration and Extermination Camp,” PLoS One, vol. 9, no. 10, 2014.

E. Mukharomah, H. Widjajanti, S. B. E. Spent, and B. Earth, “Identifikasi dan sinergisme kapang lipolitik dari limbah SBE ( Spent

Bleaching Earth ) sebagai agen bioremediasi,” vol. 13, no. 1, pp. 19–26, 2015.

T. Drevinskas, R. Mickiene, A. Maruska , M. Stankevicius, N. Tiso, J. Mikasauskaite, O. Ragazinskiene, D. Levisauskas, V. Bartkuviene, V. Snieskiene, A. Stankeviciene, C. Polcaro, E. Galli, E. Donati, T. Tekorius, O. Kornysova, and V. Kaskoniene, “Downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: Methodological approach,” Anal. Bioanal. Chem., vol. 408, no. 4, pp. 1043–1053, 2016.

H. Muchtar, Kamsina, and I. T. Anova, “Pengaruh kondisi penyimpanan terhadap pertumbuhan jamur pada gambir”, Jurnal Dinamika Penelitian Industri, vol. 22, no. 1, pp. 36–43, 2011.

J. R. Moody and T. W. Vetter, “Development of the ion exchange-gravimetric method for sodium in serum as a definitive method,” J. Res. Natl. Inst. Stand. Technol., vol. 101, no. 2, pp. 155–164, 1996.

G. S. Meena, V. S. Raina, A. K. Gupta, T. K. Mohanty, M. Bhakat, M. Abdullah and R. Bishist, “Effect of preputial washing on bacterial load and preservability of semen in Murrah buffalo bulls,” Vet. World, vol. 8, no. 6,

pp. 798–803, 2015.

X. K. Ma, N. Ding, and E. C. Peterson, “Bioaugmentation of soil contaminated with high-level crude oil through inoculation with mixed cultures including Acremonium sp.,” Biodegradation, vol. 26, no. 3, pp. 259–269, 2015.

S. L. M. Neto,P. J. Esteves, V. T. O. Santos, A. P. Paranhos, F. Cescato, V. M. Vitali, and K. M. G. Machado, “Novel salt and alkali tolerant

neotropical basidiomycetes for dye decolorisation in simulated textile effluent,” World J. Microbiol. Biotechnol., vol. 27, no. 11, pp. 2665–2673, 2011.




DOI: http://dx.doi.org/10.24845/ijfac.v3.i3.59

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